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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
PromoCell/3D Tumorsphere Medium XF
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: PromoCell
公      司:PromoCell
公司分类:
PromoCell/3D Tumorsphere Medium XF
商品介绍

The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.

Components:

The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Key features:

  • Establish 3D tumorsphere cultures directly from 2D cancer cell lines
  • Suitable for long-term routine culture of tumorspheres
  • Compatible with most commonly used cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:

TissueTested Cell LinesCell Lines Origin
BrainU-87 MGgrade IV glioblastoma / astrocytoma of the human brain
BreastMCF-7pleural effusion of metastatic human breast adenocarcinoma
BreastMDA-MB-231pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
ColonHT-29human colon adenocarcinoma
Connective tissueHT1080human fibrosarcoma
LiverHepG2hepatocellular carcinoma of the human liver
LungA-549human lung carcinoma
PancreasPanc-1epithelioid carcinoma of the human pancreatic duct
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma
SkinA-431epidermoid carcinoma of the human skin
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
Figure 4. List of some cell types successfully tested for serial passage with the PromoCell 3D Tumorsphere Medium XF.
Figure 4. List of some cell types successfully tested for serial passage with the PromoCell 3D Tumorsphere Medium XF.
品牌介绍

德国PromoCell是细胞培养产品的主要制造商,其各种人类原代细胞、干细胞和血细胞以及优化的细胞培养基尤其得到认可。全球的科学家都在使用PromoCell产品进行基础的和应用型的生物医学研究,以便从更加准确的生物模型中获得更好的实验结果。

在其旗下的PromoKine品牌中,PromoCell还提供大量成熟的细胞生物学科研产品,例如细胞分析试剂和试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISAs、细胞因子和生长因子、支原体检测试剂盒和消除试剂,以及实验室消毒剂,这些都能辅助科学家进行他们的日常实验室工作,并获得了结果。


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