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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
PromoCell/Human CD14+ Monocytes (hMoCD14+-PB)
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: PromoCell
公      司:PromoCell
公司分类:
PromoCell/Human CD14+ Monocytes (hMoCD14+-PB)
商品介绍

Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting as antigen-presenting immune cells, they can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells (moDC), macrophages, and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology.

CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes. Thus, binding of immunomagnetic particles to this antigen is employed for purification of PromoCell CD14 -Monocytes (hMoCD14 -PB) from Mononuclear Cells (MNC).

PromoCell Human CD14 Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14 Monocytes are isolated by positive selection with > 95% purity.

NEW: Our hMoCD14+ -PB are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 10.000.000 viable cells.
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density1,000,000 cells per ml
Figure 1. CD14Monocyte Cell Culture in phase contrast
Figure 1. CD14Monocyte Cell Culture in phase contrast
Figure 2. Cluster formation during differentiation of Monocytes into moDC.
Figure 2. Cluster formation during differentiation of Monocytes into moDC.
Figure 3. Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. More than 98% of the cells are negative for CD14.
Figure 3. Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. More than 98% of the cells are negative for CD14.
品牌介绍

德国PromoCell是细胞培养产品的主要制造商,其各种人类原代细胞、干细胞和血细胞以及优化的细胞培养基尤其得到认可。全球的科学家都在使用PromoCell产品进行基础的和应用型的生物医学研究,以便从更加准确的生物模型中获得更好的实验结果。

在其旗下的PromoKine品牌中,PromoCell还提供大量成熟的细胞生物学科研产品,例如细胞分析试剂和试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISAs、细胞因子和生长因子、支原体检测试剂盒和消除试剂,以及实验室消毒剂,这些都能辅助科学家进行他们的日常实验室工作,并获得了结果。


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