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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 仪器类>破碎混匀>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: PromoCell
公      司:PromoCell
公司分类:
PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
商品介绍

PromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

NEW: Our M2 Macrophages are now also available from HLA-typed donors.

Recommended plating density200,000 cells per cm2
Tested MarkersCD163 positive, CD68 positive
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
品牌介绍

德国PromoCell是细胞培养产品的主要制造商,其各种人类原代细胞、干细胞和血细胞以及优化的细胞培养基尤其得到认可。全球的科学家都在使用PromoCell产品进行基础的和应用型的生物医学研究,以便从更加准确的生物模型中获得更好的实验结果。

在其旗下的PromoKine品牌中,PromoCell还提供大量成熟的细胞生物学科研产品,例如细胞分析试剂和试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISAs、细胞因子和生长因子、支原体检测试剂盒和消除试剂,以及实验室消毒剂,这些都能辅助科学家进行他们的日常实验室工作,并获得了结果。


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