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293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
| Source | 293 (Human transformed primary embryonal kidney) |
|---|---|
| species | Human |
| Concentration | Product is supplied at a concentration of 2 mg/ml. |
| Reconstitution | During shipment, small volumes will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. |
| Stability & Storage | Store at 4°C for continuous use. Store at -20°C for three months or at -70°C for up to one year. Avoid repeated freezing and thawing (store working aliquots at -70°C). Under proper storage conditions the shelf life is 6 months from the date of receipt. |
德国PromoCell是细胞培养产品的主要制造商，其各种人类原代细胞、干细胞和血细胞以及优化的细胞培养基尤其得到认可。全球的科学家都在使用PromoCell产品进行基础的和应用型的生物医学研究，以便从更加准确的生物模型中获得更好的实验结果。
在其旗下的PromoKine品牌中，PromoCell还提供大量成熟的细胞生物学科研产品，例如细胞分析试剂和试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISAs、细胞因子和生长因子、支原体检测试剂盒和消除试剂，以及实验室消毒剂，这些都能辅助科学家进行他们的日常实验室工作，并获得了结果。
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