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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
PromoCell/Primary Cancer Culture System
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: PromoCell
公      司:PromoCell
公司分类:
PromoCell/Primary Cancer Culture System
商品介绍

PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).

Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.

Key features:

  • Perform up to five primary tumor isolations with one medium kit
  • Successfully prevent stroma overgrowth when isolating fresh tumor samples
  • Establish a culture of pure malignant cells within 4-6 weeks
  • Culture PDX tumor models in vitro
  • Defined and animal component-free formulation

Components:

  • Primary Cancer Cell Medium D-ACF, 250ml
  • SupplementMix for 250ml
  • NCCD surface treatment reagent, 2ml

The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.

Figure 1. Overview of the selective isolation of primary cancer cells from human tumor samples using the Primary Cancer Culture System. The PromoCell system makes it possible to reliably deplete non-malignant cells from the cell culture while supporting the maintenance of cancer cells and malignant cell heterogeneity. The optional establishment of cancer cell lines is indicated.
Figure 1. Overview of the selective isolation of primary cancer cells from human tumor samples using the Primary Cancer Culture System. The PromoCell system makes it possible to reliably deplete non-malignant cells from the cell culture while supporting the maintenance of cancer cells and malignant cell heterogeneity. The optional establishment of cancer cell lines is indicated.
Figure 2. Primary culture derived from a squamous cell carcinoma featuring an adherent growth pattern. The upper right inset reveals cellular heterogeneity at a greater magnification.
Figure 2. Primary culture derived from a squamous cell carcinoma featuring an adherent growth pattern. The upper right inset reveals cellular heterogeneity at a greater magnification.
Figure 3. Primary culture derived from a low-grade small cell lung cancer featuring a suspension growth pattern with 3D sphere formation.
Figure 3. Primary culture derived from a low-grade small cell lung cancer featuring a suspension growth pattern with 3D sphere formation.
品牌介绍

德国PromoCell是细胞培养产品的主要制造商,其各种人类原代细胞、干细胞和血细胞以及优化的细胞培养基尤其得到认可。全球的科学家都在使用PromoCell产品进行基础的和应用型的生物医学研究,以便从更加准确的生物模型中获得更好的实验结果。

在其旗下的PromoKine品牌中,PromoCell还提供大量成熟的细胞生物学科研产品,例如细胞分析试剂和试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISAs、细胞因子和生长因子、支原体检测试剂盒和消除试剂,以及实验室消毒剂,这些都能辅助科学家进行他们的日常实验室工作,并获得了结果。


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